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van der Walt et al. Genetic Polymorphisms of the N-acetyltransferase Genes and Risk to Parkinson's Disease

Primers and Probes used for NAT1 and NAT2 Genotyping

SNP	Change	Probe *	Sequence
445	G > A	Allele 1	tcagcctcaggtgccttgta
		Allele 2	ggatcagcctcaggtgccttgtg
		Common	tcttccgtttgacggaagagaatggattctggtat

560	G > A	Allele 1	ctagaagacagcaaatacya
		Allele 2	gggctagaagacagcaaatacyg
		Common	aaaaatctactcctttactcttaagcct

640	T > G	Allele 1	atacctgcagacatctccag
		Allele 2	tacatacctgcagacatctccat
		Common	catctgtgtttactagtaaatcattttgttccttgca

1088	T > A	Allele 1	ccatctttaaaakacatttt
		Allele 2	tcaccatctttaaaakacattta
		Common	ttattattattattattattatttgaaaaggtttgtggttatgt

1095	C > A	Allele 1	taataataataawaaatgta
		Allele 2	gcgtaataataataawaaatgtc
		Common	ttttaaagatggcctgtggttatcttggaaattggtgatttatgct

282	G > A	Allele 1	atttgttaactggagggata
		Allele 2	tgtatttgttaactggagggatg
		Common	taaaaataccctcctaacattgtggtctgaaaacc

341	T > A	Allele 1	cttctcctgcaggtgaccaa
		Allele 2	caccttctcctgcaggtgaccat
		Common	tgacggcaggaattacattgtcgatgctgggtctggaagctcctc

481	T > C	Allele 1	agagagaggaatctggtacc
		Allele 2	agaagagagaggaatctggtact
		Common	tggaccaaatcaggagagagcagtat

590	G > A	Allele 1	ttatttacgcttgaacctca
		Allele 2	gggttatttacgcttgaacctcg
		Common	aacaattgaagattttgagtctatgaatacatacc

857	G > A	Allele 1	gtgcccaaacctggtgatga
		Allele 2	ctcgtgcccaaacctggtgatgg
		Common	atcccttactatttagaataaggaacaaaataaacccttgtgta

*All PCR NAT1 products were generated using the following primers pair: F-cgatgttgggagggtatgtt and R-aaagctttctagcataaatcaccaa while NAT2 products were amplified using F-ggctgttccctttgagaacc and R-cgtgagggtagagaggatatctg. Allele specific probes were modified with either a FAM-6 or HEX florescent tag while common probes were phosphorylated on the 5� end.

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